Methyloximes have been prepared from 2-amino-2-deoxy-D-glucose, -D-mannOSe, and -o-galactose. The acetates of these derivatives yield stable compounds which are readily separated quantitatively by gas-liquid chromatography on a number of polar phases. The above compounds along with the aldononitrile
Application of nitrous acid deamination of hexosamines to the simultaneous GLC determination of neutral and amino sugars in glycoproteins
β Scribed by William H. Porter
- Book ID
- 107711660
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 920 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0003-2697
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ABSTRACr A new procedure for the quantification by g.1.c. of the carbohydrate constituents of glycoproteins is proposed which involves (a) simultaneous action of neuraminidase and neuraminic acid aldolase, (b) hydrolysis with 4~ trifluoroacetic acid at 125" for 1 h, and (c) conversion of the product
A procedure was described for the rapid determination of glucosamine and galactosamine in total acid hydrolyzate of rat renal glomerular basement membrane (GBM) by the use of amino acid analyzer. Glucosamine and galactosamine, as well as other amino acids in glycoprotein hydrolyzate, were well ident