Application of micro high-performance liquid chromatography to the separation of chiral amino acids
β Scribed by T. Takeuchi; H. Asai; Y. Hashimoto; K. Watanabe; D. Ishii
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 456 KB
- Volume
- 331
- Category
- Article
- ISSN
- 1873-3778
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π SIMILAR VOLUMES
Figure 1. Reaction of D/L-amino acids with R(Γ)-DBD-PyNCS.
All common phenyhhiohydantoin amino acids derived from sequencing are identifiable in 6 to 9 min with an isocratic method based on buffered aqueous acetonitrile, an ODS stationary phase, and programmed flow which increases during the run. With constant flow the analysis requires 16 min. The effects
A chiral liquid chromatographic method was validated to analyze the Dand L-enantiomers of five amino acids contained in a commercial solution: aspartic acid, leucine, lysine, phenylalanine, and valine. These 10 compounds were separated on a chiral crown ether column with a mobile phase composed of w