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Enantiomeric separation of five amino acids by high-performance liquid chromatography on a chiral crown ether column

✍ Scribed by François Gimenez; Mireille Soursac; Robert Farinotti

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 129 KB
Volume: 9
Category: Article
ISSN: 0899-0042
DOI: 10.1002/(sici)1520-636x(1997)9:2<150::aid-chir13>3.0.co;2-m

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✦ Synopsis

A chiral liquid chromatographic method was validated to analyze the Dand L-enantiomers of five amino acids contained in a commercial solution: aspartic acid, leucine, lysine, phenylalanine, and valine. These 10 compounds were separated on a chiral crown ether column with a mobile phase composed of water adjusted to pH 1.5 with perchloric acid, with ultraviolet detection at 220 nm. The method was applied to the commercial amino acid solution before and after sterilization by 5 kGy irradiation; no stereoconversion was observed following sterilization. Chirality 9: 150-152, 1997.

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