Enantiomeric separation of d-/l-norepinephrine and -epinephrine by high-performance liquid chromatography with a β-cyclodextrin type chiral stationary phase

✍ Scribed by Takeshi Fukushima; Katsuhisa Murayama; Tomofumi Santa; Hiroshi Homma; Kazuhiro Imai

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 61 KB
• Volume: 12
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199801/02)12:1<1::aid-bmc694>3.0.co;2-w

✦ Synopsis

Enantiomeric separation of d-/l-norepinephrine (NE) and d-/l-epinephrine (E) was investigated with various mobile phases by high-performance liquid chromatography using a ␤-cyclodextrin type chiral stationary phase as a chiral column. The mobile phase of 2.5 M phosphate buffer (pH 3.0) gave separation factors (␣ s) of 1.04 and 1.06 for NE and E, respectively.