Effects of glycerol on the quality of ground and whole pancreas stored frozen at -30 "C were studied. The quality was assessed from quantitative changes in soluble proteins, proteolytic enzyme activity, and insulin content. Grinding was found to enhance the effects of the cryoprotector due to its u
Application of glycerol to freezing bovine pancreas. Part 1. Effect of cryoprotector on activity of certain enzymes in frozen minced pancreas
โ Scribed by Hajduk, E. ;Fik, M.
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 307 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0027-769X
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โฆ Synopsis
Effects of different concentrations of glycerol on the activity of amylase, lipase, and proteases in frozen minced pancreas were studied. A 15 % glycerol addition was found to decrease the proteolytic enzyme activity and to increase the activity of amylase. Moreover, accelerated freezing was shown to enhance the activity of all the enzymes studied.
๐ SIMILAR VOLUMES
## Abstract Changes in protein solubility, amino nitrogen and activity of proteases in bovine pancreas frozen at โ70 and โ18ยฐC after soaking in various aqueous glycerol solutions were studied. Reduction of freezing temperature causes decrease of value in all studied parameters. Additionally, an inh