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Application of glycerol to freezing bovine pancreas. Part 1. Effect of cryoprotector on activity of certain enzymes in frozen minced pancreas

โœ Scribed by Hajduk, E. ;Fik, M.


Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
307 KB
Volume
34
Category
Article
ISSN
0027-769X

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โœฆ Synopsis


Effects of different concentrations of glycerol on the activity of amylase, lipase, and proteases in frozen minced pancreas were studied. A 15 % glycerol addition was found to decrease the proteolytic enzyme activity and to increase the activity of amylase. Moreover, accelerated freezing was shown to enhance the activity of all the enzymes studied.


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## Abstract Changes in protein solubility, amino nitrogen and activity of proteases in bovine pancreas frozen at โˆ’70 and โˆ’18ยฐC after soaking in various aqueous glycerol solutions were studied. Reduction of freezing temperature causes decrease of value in all studied parameters. Additionally, an inh