Application of continuous zone electrophoresis to preparative separation of proteins
✍ Scribed by Sunil Nath; Horst Schütte; Helmut Hustedt; Wolf-Dieter Deckwer
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 585 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0006-3592
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We have described modifications of apparatus and technique of preparative sodium dodecyl sulfate-gel electrophoresis which improve the resolution of very large proteins. These modifications permit the resolution of several components of apolipoprotein B from human blood plasma.
## Abstract In CZE, the adsorption of the proteins on the capillary wall is a common problem. This paper describes the simple method of utilizing zwitterionic buffer additives to improve the separation of proteins in untreated fused silica capillaries at neutral pH. Three kinds of zwitterion are ev