✦ LIBER ✦

Application of Active Charcoal for the Purification of Purine and Pyrimidine Derivatives in Milk for Thin Layer Chromatography Analysis

✍ Scribed by Rashid, R.

Publisher: John Wiley and Sons
Year: 1973
Tongue: English
Weight: 259 KB
Volume: 17
Category: Article
ISSN: 0027-769X
DOI: 10.1002/food.19730170503

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✦ Synopsis

Abstract

A method has been developed to get purified purine and pyrimidine derivatives content of milk except uric acid by deproteinization with TCA, extraction of ether‐soluble materials and treatment with active charcoal by adsorption‐desorption cycle. The conditions and the nature of the charcoal have been discussed. Uric acid could not be recovered due to its low solubility and complete retention on the charcoal. Total recovery based on the original extract was around 80% and over 90% according to the adsorbed materials.

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