Thin-layer chromatography and high resolution selected ion monitoring for the analysis of C19 steroids in human hyperplastic prostate tissue
✍ Scribed by D. S. Millington; M. E. Buoy; G. Brooks; M. E. Harper; K. Griffiths
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- English
- Weight
- 461 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1076-5174
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✦ Synopsis
Interfering nonpolar lipid material was removed from the acetone extracts of several samples of benign hyperplastic prostate tissue. Endogenous steroids were separated by preparative thin-layer chromatography into fractions contahing testosterone, Sa-dihydrotestosterone and the 5a-androstane-3,17-diols, and concentrations of these steroids were measured by molecular ion monitoring of suitable derivatives at a resolution of 8500 to 10 OOO during combined gas chromatography mass spectrometry. The sensitivity and specificity of this procedure allowed unequivocal detection of the steroids of interest at the femtomole level and the incorporation of epimers as internal standards enabled accurate quantitative measurements. The results indicated that 5a-dihydrotestosterone is usually the most predominant of the steroids measured, and that of the 5aandrostanediols, only the 3a, 17p and 3P,17p isomers occur in measurable concentration, with the former usually predominant.