Application of overpressured layer chromatography in red pepper analysis. Study of the carotenoids responsible for the red color in ground red pepper
✍ Scribed by Aczél, A.
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 170 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
soaking, provided thesample solvent and the stationary phase are References similar in polarity. Perfectly shaped peaks with areas representing the full amount of solute material are obtained. However, if the sample volume injected exceeds a critical volume, in the case of toluene in n-pentane it was around 5 pl, reconcentration is no longer complete. Peaks are distorted; in some cases distortion is obvious; more often, however, peaks are just of somewhat reduced size.
The problem can be solved either by selection of a solvent providing full trapping (high retention power of the solute in the solvent being important) or by calibration involving injection of volumes corresponding to those used for the analyses.