Antigenic characteristics of the complete and truncated capsid protein VP1 of enterovirus 71
โ Scribed by Jianhua Zhang; Min Dong; Bingfu Jiang; Xing Dai; Jihong Meng
- Book ID
- 116968850
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 632 KB
- Volume
- 167
- Category
- Article
- ISSN
- 0168-1702
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## Abstract The homology modeling technique has been used to construct the structure of enterovirus 71 (EV 71) capsid protein VP1. The protein is consisted of 297 amino acid residues and treated as the target. The amino acid sequence identity between the target protein and sequences of template pro
To produce enterovirus 71 antigen for diagnostic purposes, the gene encoding the entire capsid protein VP1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and expressed in Escherichia coli as a polyhistidine fusion protein. Western blotting experiments with sera fro