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Characterization of the enterovirus 71 VP1 protein as a vaccine candidate

โœ Scribed by Zhou, Shi-Li; Ying, Xiao-Ling; Han, Xue; Sun, Xian-Xun; Jin, Qi; Yang, Fan


Book ID
126471075
Publisher
John Wiley and Sons
Year
2014
Tongue
English
Weight
200 KB
Volume
87
Category
Article
ISSN
0146-6615

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To produce enterovirus 71 antigen for diagnostic purposes, the gene encoding the entire capsid protein VP1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and expressed in Escherichia coli as a polyhistidine fusion protein. Western blotting experiments with sera fro