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Expression and immunogenicity of novel subunit enterovirus 71 VP1 antigens

โœ Scribed by Juan Xu; Shixia Wang; Weihua Gan; Wenhong Zhang; Liwen Ju; Zuhu Huang; Shan Lu


Book ID
116303221
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
915 KB
Volume
420
Category
Article
ISSN
0006-291X

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Expression of caspid protein VP1 for use
โœ Shin-Ru Shih; Yi-Shuane Li; Chiuan-Chian Chiou; Pin-Chau Suen; Tzou-Yien Lin; Lu ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 916 KB

To produce enterovirus 71 antigen for diagnostic purposes, the gene encoding the entire capsid protein VP1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and expressed in Escherichia coli as a polyhistidine fusion protein. Western blotting experiments with sera fro