Animal Cell Technology || In-Vitro Toxicological Effects of Pluronic F-68
✍ Scribed by Carrondo, Manuel J. T.; Griffiths, Bryan; Moreira, José L. P.
- Book ID
- 120180983
- Publisher
- Springer Netherlands
- Year
- 1997
- Weight
- 876 KB
- Category
- Article
- ISBN
- 940115404X
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The comparative effects of Pluronic on cell growth of hybridomas were studied. The addition of 0.05% Pluronic decreased the steady-state cell number in continuous culture by about 12% compared to a non-supplemented culture. In short-term experiments, results demonstrated a gradual decrease in cell n
## Abstract The surfactant Pluronic F‐68 (PF‐68) is widely used in large‐scale mammalian cell culture to protect cells from shear stress that arises from agitation and gas sparging. Several studies suggested that PF‐68 is incorporated into the cell plasma membrane and could enter the cells, but wit