Analytical-Band Centrifugation of an Active Enzyme Substrate Complex : 2. Determination of Active Units of Various Enzymes
✍ Scribed by René Cohen; Michel MireÅ
- Book ID
- 115112907
- Publisher
- John Wiley and Sons
- Year
- 1971
- Tongue
- English
- Weight
- 581 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1432-1327
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The active eiizyme centrifugat.ion (AEC) method preseiited here permits the hydrodynamic study of active enzyme-substrate(s) complexes in solutions containing microgram am0unt.s of the studied enzyme, even if the enzyme preparation is very impure. The AEC method can be used only when the specific en
We developed a simple, kinetic method for the determination of catalase activity in which i) the enzyme catalyzes the peroxidation of ethanol by hydrogen peroxide to acetaldehyde and water, and ii) the acetaldehyde so formed is rapidly oxidized to acetic acid and NADPH by the addition of an excess o