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Analytical-Band Centrifugation of an Active Enzyme-Substrate Complex : 1. Principle and Practice of the Centrifugation

✍ Scribed by René Cohen; Michel Mire

Book ID
115112906
Publisher
John Wiley and Sons
Year
1971
Tongue
English
Weight
772 KB
Volume
23
Category
Article
ISSN
1432-1327

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📜 SIMILAR VOLUMES

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The active eiizyme centrifugat.ion (AEC) method preseiited here permits the hydrodynamic study of active enzyme-substrate(s) complexes in solutions containing microgram am0unt.s of the studied enzyme, even if the enzyme preparation is very impure. The AEC method can be used only when the specific en

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The mechanism of an enzymatic reaction is better understood when the structures of ground and transition states are known. We have set out to determine enzymesubstrate (ES) and transition state (TS) for the S N 2 displacement of Cl Ϫ from 1,2dichlorethane (DCE) by Asp 124-CO Ϫ 2 at the active site o

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