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Analysis of tRNA population from Drosophila melanogaster by means of polyacrylamide gel mapping

✍ Scribed by Andŕe Mazabraud; Jean-Pierre Garel

Book ID
115910043
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
624 KB
Volume
105
Category
Article
ISSN
0014-5793

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✍ Schmidt, Thomas ;Egg, Albert H. ;Kubli, Eric 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 560 KB

Transfer RNAAsp2delta was isolated from Drosophila melanogaster by affinity chromatography on concanavalin A-Sepharose. The tRNA was iodinated "in vitro" with Na [125I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the