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Analysis of thiohydantoins from amino acids by gas-liquid chromatography on short glass capillary columns

✍ Scribed by Julius Eyem; John Sjöquist

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
844 KB
Volume
52
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method for separation of amino acid methyl or phenyl thiohydantoins by GLC on a short glass capillary column is described. Calculations of required parameters of the capillary column are presented. By the described methods, nineteen of twenty silylated methylthiohydantoins were separated in one run. The last one (histidine) can be identified on the same column by starting the analysis at a higher temperature. Cysteine and arginine were analysed as S-methylcysteine and ornithine, respectively.

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The phenylthiohydantoins (Pth) of the common amino acids can be resolved in a single analysis using a 25 x 0.46cm DuPont Zorbax cyanopropylsilane (CN) column developed with a gradient of methanol/acetonitrile (17:3) in sodium acetate buffer, pH 5.4. The Zorbax CN columns exhibit greater durability,