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Analysis of the major polypeptides of spectrin by tryptic digestion

✍ Scribed by Maddy, Alun H. ;Dunn, Michael J.

Publisher
Wiley (John Wiley & Sons)
Year
1978
Tongue
English
Weight
767 KB
Volume
8
Category
Article
ISSN
0091-7419

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✦ Synopsis

Abstract

The two major polypeptides of erythrocyte membrane spectrin have been isolated by preparative polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The tryptic peptide maps of the two polypeptides have been prepared by thin‐layer chromatography and electrophoresis. Radioactive peptides have been prepared by ^14^C‐carboxymethylation and chloramine T‐catalysed ^125^I iodination. Maps of both sets of peptides demonstrate a marked similarity between the two parent polypeptides.

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