✦ LIBER ✦
Elevated temperature–extended column length conventional liquid chromatography to increase peak capacity for the analysis of tryptic digests
✍ Scribed by Pat Sandra; Gerd Vanhoenacker
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 332 KB
- Volume
- 30
- Category
- Article
- ISSN
- 1615-9306
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
High efficiency separations (200 000 plates) were obtained on conventional LC equipment by coupling 8×25 cm×2.1 (or 4.6) mm id×5 μm d~p~ ODS columns (total length 2 m) and operation at 60°C using a dedicated LC oven. The peak capacity in this 1‐D set‐up was 900 for the separation of human serum tryptic peptides analyzed after depletion of six highly abundant proteins. The chromatographic performance of an elevated temperature–extended column length conventional LC is highlighted.