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Analysis of radioactive and nonradioactive purine bases, nucleosides, and nucleotides by high-speed chromatography on a single column

✍ Scribed by Bohdan Bakay; Erkki Nissinen; Lawrence Sweetman

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 769 KB
Volume: 86
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90319-6

No coin nor oath required. For personal study only.

✦ Synopsis

The concentrations of radioactive and nonradioactive purine bases, purine nucleosides, purine mono-, di-, and trinucleotides in acid extracts of fibroblasts were determined by anion-exchange column chromatography. The concentrations ofnonradioactive components were determined by computerized integration of the signal from a double-beam uv-detector. The radioactive metabolites were quantitated by high-efficiency, continuous liquid scintillation counting, employing a discrete sample transport system.

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