๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Analysis of metabolic control In Vivo using molecular genetics

โœ Scribed by Kevin M. Brindle


Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
742 KB
Volume
14
Category
Article
ISSN
0263-6484

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


Studies of metabolic control using NMR a
โœ Kevin M. Brindle; Sandra M. Fulton; Helen Gillham; Simon-Peter Williams ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 147 KB ๐Ÿ‘ 2 views

The techniques of NMR spectroscopy and molecular genetics have provided new and powerful approaches to studying the control and organisation of cellular metabolism in vivo. We review here our recent applications of these methodologies to the study of energy metabolism in yeast and mammalian cells.

Enzymology in vivo using NMR and molecul
โœ Kevin M. Brindle; Alexandra M. Fulton; Simon-Peter Williams ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 827 KB

## Abstract Models of metabolic flux regulation are frequently based on an extrapolation of the kinetic properties of enzymes measured __in vitro__ to the intact cell. Such an extrapolation assumes a detailed knowledge of the intracellular environment of these enzymes in terms of their free substat

Molecular analysis of genetic changes in
โœ Emilia K. Bijlsma; Annet M. J. Voesten; Engelien H. Bijleveld; Dirk Troost; Andr ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 488 KB

Ependymomas are glial cell-derived tumors. They are, in contrast t o other gliomas (astrocytomas, oligodendrogliomas, and oligoastrocytomas), ill-defined with respect t o the genes and chromosomal segments important in their tumorigenesis. In this study, we extensively screened I 7 ependymomas for g

Use and Limitations of Modular Metabolic
โœ Stefan Schuster ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 201 KB

Metabolic Control Analysis (MCA), it has been shown that control on flux is in most cases shared by several enzymes rather than concentrated on one ``rate-limiting step.'' This analysis also allows the quantification of the control exerted by groups (modules) of enzymes. The modules may correspond t

Control Analysis in Terms of Generalized
โœ Stefan Schuster ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 244 KB

Metabolic Control Analysis had originally been devised to quantify the effects of changes in enzyme concentrations on steady-state fluxes and metabolite concentrations. In many situations, fluxes and concentrations are not the only relevant variables. A formalism is presented by which the control of

In vivo analysis of metabolic dynamics i
โœ Uwe Theobald; Werner Mailinger; Michael Baltes; Manfred Rizzi; Matthias Reuss ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 468 KB ๐Ÿ‘ 2 views

The goal of this work was to obtain rapid sampling technique to measure transient metabolites in vivo. First, a pulse of glucose was added to a culture of the yeast Saccharomyces cerevisiae growing aerobically under glucose limitation. Next, samples were removed at 2 to 5 s intervals and quenched us