Analysis of lymphocyte activation and metabolism by flow cytometry
β Scribed by Carl H. June
- Book ID
- 103972842
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 710 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0952-7915
No coin nor oath required. For personal study only.
β¦ Synopsis
The use of flow cytometry for the study of lymphocyte activation and cellular metabolism continues to expand. This has permitted rapid progress in the understanding of the regulation of multiple intracellular ions, metabolic pathways, and the control of gene expression. Knowledge of these basic mechanisms of cellular homeostasis is now being applied to the flow cytometric study of immune dysregulation. Various flow cytometric techniques for measuring apoptosis are summarized.
π SIMILAR VOLUMES
## Abstract A method of preparation and flow cytometric analysis of chromosomes from human peripheral lymphocytes is described. The procedure allows a resolution coefficient of variation better than 3% using propidium iodide staining and a commercially available flow cytometer.
Development of a more cost-effective and efficient method of performing lymphocyte subset analysis is of continuing importance in clinical flow cytometry laboratories. Current two-color methods utilize forward and right angle light scatter and multiple tubes per sample and are thereby liable to gate