Analysis of Lipids in Crude Extracts by 13C Nuclear Magnetic Resonance

✍ Scribed by P. Pollesello; R. Toffanin; O. Eriksson; I. Kilpelainen; P.H. Hynninen; S. Paoletti; N.E.L. Saris

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 608 KB
• Volume: 214
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1993.1483

✦ Synopsis

Phospho- and glycolipids with mono- or polyunsaturated fatty acid chains, free and acylated steroids, carotenes and carotenoids, chlorophylls, and related pheophytins were detected in crude extracts by NMR spectroscopy without any need for prior separation. A broad range of molecules belonging to different lipid classes could be identified in one-dimensional ({ }^{13} \mathrm{C}) NMR spectra obtained by applying a polarization transfer pulse sequence (DEPT (135^{\circ}) ). Directly detected or ({ }^{1} \mathrm{H}-) detected two-dimensional heterocorrelated NMR experiments were performed to facilitate the assignment of peaks arising from carotenoids, unsaturated fatty acid chains, and chlorophylls. ({ }^{13} \mathrm{C}) NMR data of crude lipid extracts from the macroalgae Ulva rigida and Fucus virsoides are shown to yield an informative overview of their lipid content. NMR is thus proposed as a simple, nonselective, and nondestructive technique for the first screening of the main lipid classes in complex lipid mixtures. (c) 1993 Academic Press, Inc.