Liquid Chromatography/Ultra Violet/Mass Spectrometric and Liquid Chromatography/Nuclear Magnetic Resonance Spectroscopic Analysis of Crude Extracts of Gentianaceae Species

✍ Scribed by Jean-Luc Wolfender; Sylvain Rodriguez; Kurt Hostettmann; Wolf Hiller

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 195 KB
• Volume: 8
• Category: Article
• ISSN: 0958-0344
• DOI: 10.1002/(sici)1099-1565(199705)8:3<97::aid-pca350>3.0.co;2-z

✦ Synopsis

High performance liquid chromatography (HPLC) coupled with ultra violet photodiode array (LC/UV-DAD) and mass spectrometry (LC/MS) detection has been used routinely for the chemical screening of crude extracts of Gentianaceae species. The recent progress realised in the field of nuclear magnetic resonance spectroscopy (NMR) has given a new impulse to the coupling of this detection method with liquid chromatography (LC/NMR) and several LC/NMR probes are now commercially available. In order to evaluate the potential of this coupled technique as a complement to LC/UV/MS for the on-line identification of plant metabolites, two characteristic Gentianaceae species were analysed, namely Swertia calycina and Gentiana ottonis. On-line UV, MS, MS/MS 1 H-NMR and 1 H-1 H-NMR spectra of the major constituents of these two plants allowed their identification directly within the crude extracts. The potential and the limitations of LC/NMR used together with LC/UV/MS for the on-line analysis of plant metabolites are discussed.