𝔖 Bobbio Scriptorium
✦   LIBER   ✦

13C-Isotopic Enrichment of Glutathione in Cell Extracts Determined by Nuclear Magnetic Resonance Spectroscopy

✍ Scribed by Michael P. Gamcsik

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
83 KB
Volume
266
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

An NMR method was developed for measuring the isotopic enrichment of glutathione in extracts of cells fed a medium containing [3,3-13 C 2 ]cystine. Two sublines of human mammary adenocarcinoma MCF-7 cells were exposed to growth medium containing the labeled cystine for varying periods, treated with monobromobimane, harvested, and extracted with perchloric acid. The glutathione-bimane adduct was partially purified by solid-phase extraction before analysis by 1 H NMR spectroscopy. The isotopic enrichment of the ␤-carbon of the cysteinyl residue of glutathione was determined directly in the cell extracts without further purification. These isotopic enrichment data can be used to determine the rate of synthesis of glutathione in cell and tissue extracts.

📜 SIMILAR VOLUMES

Accurate Determination of13C Enrichments
Accurate Determination of13C Enrichments in Nonprotonated Carbon Atoms of Isotopically Enriched Amino Acids by1H Nuclear Magnetic Resonance
✍ Volker F. Wendisch; Albert A. de Graaf; Hermann Sahm 📂 Article 📅 1997 🏛 Elsevier Science 🌐 English ⚖ 113 KB

A method for the accurate determination of 13C enrichments in nonprotonated carbon atoms of organic compounds that makes use of unresolved 13C satellites of proton(s) bonded to the vicinal carbon atom was developed. Using glutamate as a model molecule, this 1H nuclear magnetic resonance (NMR) invers

13C nuclear magnetic resonance spectrosc
13C nuclear magnetic resonance spectroscopy of glucose metabolism in human red blood cells
✍ Geetha Ghai; Nina C. Gonnella 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 277 KB

Glucose metabolism of human red blood cells was investigated using carbon-13 nuclear magnetic resonance spectroscopy under both oxygenated and nonoxygenated conditions. These results show that under oxygenated conditions reversal of 3-phosphoglyceraldehyde to glucose is in competition with its catab

Method for Determination of Intracellula
Method for Determination of Intracellular Sodium in Perfused Cancer Cells by 23Na Nuclear Magnetic Resonance Spectroscopy
✍ L.L. Hansen; J. Rasmussen; E. Friche; J.W. Jaroszewski 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 384 KB

Changes of intracellular sodium concentrations are often an indication of disease or malfunction. In this work, shift reagent-aided 23Na NMR spectroscopic determination of intracellular sodium was adapted to measurements with perfused cells embedded in agarose gel threads. Ehrlich ascites tumor cell

Identification and Quantitation of Phosp
Identification and Quantitation of Phosphorus Metabolites in Yeast Neutral pH Extracts by Nuclear Magnetic Resonance Spectroscopy
✍ Anita Teleman; Peter Richard; Mervi Toivari; Merja Penttilä 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 114 KB

31 P NMR spectroscopy offers a possibility to obtain a survey of all low-molecular-weight phosphorylated compounds in yeast. The yeast cells have been extracted using chloroform into a neutral aqueous phase. The use of high fields and the neutral pH extracts, which are suitable for NMR analysis, res

Deuterium-induced 13C nuclear magnetic r
Deuterium-induced 13C nuclear magnetic resonance isotope shifts and 13C2H couplings for signal assignments and determination of deuteration site in cyclooctanone
✍ Slobodan Milosavljević; Dragoslav Jeremić; Mihailo Lj. Mihailović; Felix W. Wehr 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 158 KB

## Abstract Unambiguous signal assignments in the ^13^C spectra of monodeuterated cyclooctanones derived from the lead tetraacetate or silver oxide/bromine oxidations of the corresponding 1‐monodeutero‐alcohols have been obtained from deuterium‐induced ^13^C isotope shifts and geminal and vicinal ^