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Analysis of insect triacylglycerols using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry

✍ Scribed by Edita Kofroňová; Josef Cvačka; Pavel Jiroš; David Sýkora; Irena Valterová

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
355 KB
Volume
111
Category
Article
ISSN
1438-7697

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✦ Synopsis

Abstract

An HPLC non‐aqueous reversed‐phase separation system was adapted for analyzing insect triacylglycerols (TAG). The method uses two conventional Nova‐Pak C18 columns connected in series, for a total length of 45 cm. The mobile phase gradient is mixed from acetonitrile and 2‐propanol, and the flow rate is programmed from 1.0 to 0.7 mL/min. TAG are detected by atmospheric pressure chemical ionization‐mass spectrometry. The method ensures efficient separation of isomers and analysis of high‐molecular‐weight TAG with equivalent chain lengths up to 72. The method performance is demonstrated on analysis of TAG isolated from the fat body of the bumblebee Bombus lucorum.

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