The technique of high-pH anion-exchange chromatography with pulsed amperometric detection has recently been shown to be a powerful method for resolving closely related oligosaccharides [M. R. Hardy and R. R. Townsend, Proc. N&l. Acad. Sci. U.S. A., 85 (1988) 3289932931. This report describes separat
Analysis of glycosphingolipid-derived oligosaccharides by high pH anion exchange chromatography
โ Scribed by Shutian Ruan; Kenneth O. Lloyd
- Publisher
- Springer US
- Year
- 1994
- Tongue
- English
- Weight
- 623 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1573-4986
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Oligosaccharides released enzymatically by N-glycanase from fetuin, alpha-acid glycoprotein, human chorionic gonadotropin, platelet-derived growth factor, and kallikrein were chromatographed on a polymeric pellicular anion-exchange column at pH values of 5 and 13. Separations occurred into groups of