Analysis of O-Linked Oligosaccharide Alditols by High-pH Anion-Exchange Chromatography with Pulsed Amperometric Detection
โ Scribed by Norihiro Kotani; Seiichi Takasaki
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 150 KB
- Volume
- 252
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
To establish high-pH anion-exchange chromatography with fluorometric detection (HPAEC-FD) for the sensitive oligosaccharide analysis, the system for HPAEC with pulsed amperometric detection (PAD) was modified by placing the anion micromembrane suppressor between PAD and fluoromonitor to neutralize t
A simple one-step method for the analysis of monosaccharides including galactosaminitol after acidic hydrolysis is described. The hydrolyzate was re- \(\mathrm{N}\)-acetylated and analyzed by high-performance anion-exchange chromatography and the sugars were detected by a pulsed amperometric detecto
An assay has been developed to quantitate the amount of mannose 6-phosphate in glycoproteins using high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The method was tested on a recombinant lysosomal enzyme, human alpha-galactosidase A, that contains mannose 6-phosp