Improvements to in-Line Desalting of Oligosaccharides Separated by High-pH Anion Exchange Chromatography with Pulsed Amperometric Detection
β Scribed by James R. Thayer; Jeffrey S. Rohrer; Nebojsa Avdalovic; R.Patrick Gearing
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 175 KB
- Volume
- 256
- Category
- Article
- ISSN
- 0003-2697
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An assay has been developed to quantitate the amount of mannose 6-phosphate in glycoproteins using high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The method was tested on a recombinant lysosomal enzyme, human alpha-galactosidase A, that contains mannose 6-phosp
To establish high-pH anion-exchange chromatography with fluorometric detection (HPAEC-FD) for the sensitive oligosaccharide analysis, the system for HPAEC with pulsed amperometric detection (PAD) was modified by placing the anion micromembrane suppressor between PAD and fluoromonitor to neutralize t