To establish high-pH anion-exchange chromatography with fluorometric detection (HPAEC-FD) for the sensitive oligosaccharide analysis, the system for HPAEC with pulsed amperometric detection (PAD) was modified by placing the anion micromembrane suppressor between PAD and fluoromonitor to neutralize t
The resolution of oligosaccharides by high pH anion exchange chromatography
โ Scribed by John M. McGuire; Moira A. Elliott; Heather G. Elliott; Kevin D. Smith
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 278 KB
- Volume
- 270
- Category
- Article
- ISSN
- 0008-6215
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Fucosylated oligosaccharides of the /3Gal(l+4)GlcNAc-, PGal(l+4)Glc-, and /3Gal(l+3)GlcNAc+eries were chromatographed on a high-performance anion-exchange pellicular resin under alkaline conditions (pH ~13). Fucosylation of either lactose, lactosamine (Type II chains), or lacto-N-biose (Type I chain