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Analysis of Glycoproteins Separated by Two-Dimensional Gel Electrophoresis Using Lectin Blotting Revealed by Chemiluminescence

✍ Scribed by P. Gravel; O. Golaz; C. Walzer; D.F. Hochstrasser; H. Turler; L.P. Balant

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 839 KB
Volume: 221
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1994.1380

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✦ Synopsis

The carbohydrate structures of blotted glycoproteins can be analyzed by probing with lectins. The objective of the present work was to optimize the lectin blotting of human plasma glycoproteins separated by two-dimensional gel electrophoresis and the detection by the sensitive chemiluminescence method. The proposed detection method was found to be ten times more sensitive than a standard colorimetric reaction. Furthermore, the generated signals are detected on a X-ray film and provide a permanent record. The method is also very reliable when compared to the colorimetric detection. The present procedure for glycoprotein analysis is particularly well suited for screening changes in glycosylation of proteins in biological samples.

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