Television-based densitometric analysis of proteins separated by two-dimensional gel electrophoresis

✍ Scribed by B.F. Aycock; D.E. Weil; D.V. Sinicropi; D.L. McIlwain

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 875 KB
• Volume: 14
• Category: Article
• ISSN: 0010-4809
• DOI: 10.1016/0010-4809(81)90003-3

✦ Synopsis

A program is described for a computer-aided, television-based system which can quantify the densities of stained proteins on two-dimensional electrophoretic gels. The system is designed to detect and graphically reproduce the edges of spots which are often located in nonuniform levels of background stain and to integrate the density of each spot detected. Tests of the system are described which show that it responds linearly to optical densities encountered on the electrophoretic gels and is sulliciently stable and sensitive to permit quantitative analysis of most, if not all, proteins separated on a gel.