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Analysis of Foreign Protein Overproduction in Recombinant CHO Cells : Effect of Growth Kinetics and Cell Cycle Traverse

✍ Scribed by MAN BOCK GU; PAUL TODD; DHINAKAR S. KOMPALA

Book ID
119871109
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
609 KB
Volume
721
Category
Article
ISSN
0890-6564

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## Abstract Human NHIK 3025 cells, synchronized by mitotic selection, were given 2 mM thymidine, which inhibited DNA synthesis without reducing the rate of protein accumulation. After removal of the thymidine the cells proceeded towards mitosis and cell division, with an S duration 2 hours shorter