Analysis of demethylaminoazobenzene-thiohydantoins of amino acid by high-pressure liquid chromatography

✍ Scribed by J.Y. Chang; A. Lehmann; B. Wittmann-Liebold

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 288 KB
• Volume: 102
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90171-2

✦ Synopsis

Dimethylaminoazobenzene-thiohydantoins

of amino acid can be quantitatively analyzed by high-pressure liquid chromatography at picomole level. As little as 5 to 10 pmol of dimethylaminoazobenzene-thiohydantoins of amino acid can easily be detected in the visible region (436 nm) against a stable baseline. Three amino acid pairs, namely glutamine and threonine, methionine and proline, and leucine and isoleucine, have not yet been separated. This new technique provides a sensitive and efficient tool for measuring the recovery of amino terminal amino acids using the dimethylaminoazobenzene-isothiocyanate method and the repetitive yield of sequence determination using the dimethylaminoazobenzene-isothiocyanate phenylisothiocyanate double-coupling method.