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Analysis of cypridina luciferin for nitrogen

✍ Scribed by Chase, Aurin M. ;Gregg, James H.


Publisher
Wiley (John Wiley & Sons)
Year
1949
Tongue
English
Weight
261 KB
Volume
33
Category
Article
ISSN
0095-9898

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Cypridina bioluminescence I Sructure of
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BIOLlEilNlSCENCE of Cypridina hilgendorfii (Japenese name:umi-hotaru) has been studied extensively since 1917, when Harvey (1) observed the luciferin-luciferase reaction with extracts of this animal. The luminescent system of Cypridina is one of the simplest among luminous animals and plantc 60 far

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## T W O FIGURES Sodiuni azide is known to inhibit a number of reactions involving enzynies (e.g. : Keilin, '33, '36 ; Stannard, '39 ; Armstrong and Fisher, '40). Giese and Fisher (unpublished data) have found that the luminescence of a species of luminous bacteria is depressed by sodium azide and

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## THREE FIGURES Cyanide is known to retard cellular oxidations by inactivation of some of the enzymes concerned. Since luminescence in Cypridina results when luciferin is oxidized in the presence of luciferase, cyanide might be expected t o affect luciferase with consequent reduction of luminesce