The effect of cyanide on cypridina luciferin
โ Scribed by Giese, Arthur C. ;Chase, Aurin M.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1940
- Tongue
- English
- Weight
- 555 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0095-9898
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โฆ Synopsis
THREE FIGURES
Cyanide is known to retard cellular oxidations by inactivation of some of the enzymes concerned. Since luminescence in Cypridina results when luciferin is oxidized in the presence of luciferase, cyanide might be expected t o affect luciferase with consequent reduction of luminescence. Harvey ('17) and Van Schouwenburg ('38) found no visible effect of cyanide on a glowing extract of whole Cypsidina. Such a mixture, however, contains besides luciferin and luciferase, many impnrities which might selectively bind the cyanide. Experiments were, therefore, designed to study the effect of cyanide on partially purified luciferin and dialysed luciferase.
๐ SIMILAR VOLUMES
## T W O FIGURES Sodiuni azide is known to inhibit a number of reactions involving enzynies (e.g. : Keilin, '33, '36 ; Stannard, '39 ; Armstrong and Fisher, '40). Giese and Fisher (unpublished data) have found that the luminescence of a species of luminous bacteria is depressed by sodium azide and
Although luminescence of Cypridina lucif erin by oxidants mhcn dissolved in ethyl alcohol has been observed, no conditions have been previously found under which Cypridina luciferin will emit light in aqueous solutions in the absence of Cypridina luciferase. All attempts to observe luminescence duri