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Analysis of brain mRNA by reverse transcription-polymerase chain reaction and hybridization with digoxigenin-labeled DNA probe

✍ Scribed by Marie-Madeleine Gabellec; Rémy Griffais; Gilles Fillion; France Haour


Book ID
117768999
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
307 KB
Volume
1
Category
Article
ISSN
1385-299X

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This study was carried out to determine whether Y-bearing porcine spermatozoa could be detected by in situ hybridization using a digoxigenin (Dig)-labelled DNA probe specific to the Y chromosome produced by polymerase chain reaction (PCR). A conventional PCR (with Dig-dUTP) was performed using a set