Detection of Y-bearing porcine spermatoz
✍
Tatsuo Kawarasaki; Tetsuya Kohsaka; Masaru Sone; Mitsutoshi Yoshida; Kimio Bamba
📂
Article
📅
1995
🏛
John Wiley and Sons
🌐
English
⚖ 492 KB
This study was carried out to determine whether Y-bearing porcine spermatozoa could be detected by in situ hybridization using a digoxigenin (Dig)-labelled DNA probe specific to the Y chromosome produced by polymerase chain reaction (PCR). A conventional PCR (with Dig-dUTP) was performed using a set