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Detection of HIV-1 DNA by polymerase chain reaction incorporation of digoxigenin-11-dUTP and hybridization to immobilized probes

✍ Scribed by N.Costa Taveira; P.C. Gomes; M.O. Santos Ferreira; J.Moniz Pereira


Book ID
115638038
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
351 KB
Volume
8
Category
Article
ISSN
0890-8508

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Detection of Y-bearing porcine spermatoz
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This study was carried out to determine whether Y-bearing porcine spermatozoa could be detected by in situ hybridization using a digoxigenin (Dig)-labelled DNA probe specific to the Y chromosome produced by polymerase chain reaction (PCR). A conventional PCR (with Dig-dUTP) was performed using a set