## Abstract Direct rotavirus serotyping (VP7, G type) in stool specimens was carried out by reverse transcription and polymerase chain reaction amplification (RTβPCR) and compared to serotyping by enzyme immunoassay with monoclonal antibodies (EIAβMAb). The methods used for doubleβstranded (ds) RNA
Amplification, detection, and automated sequencing of gibbon interleukin-2 mRNA by Thermus aquaticus DNA polymerase reverse transcription and polymerase chain reaction
β Scribed by A.L. Shaffer; W. Wojnar; W. Nelson
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 622 KB
- Volume
- 190
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Detection of illegitimate transcripts of prostate-specific antigen
Reverse transcriptase-polymerase chain reaction (RT-PCR) targeted at keratin 19 mRNA was applied to detect circulating cancer cells in the peripheral and portal blood of pancreatic and gastric cancer patients. Keratin 19 mRNA expression was studied by RT-PCR in cancer tissues (12 pancreatic and 15 g
A procedure for amplifying and detecting nucleic acid sequences in situ using cells in suspension and flow cytometry has been developed. The process involves the use of the polymerase chain reaction (PCR) and a fluorescent in situ hybridization (FISH) protocol developed in our laboratory to detect t
To examine the relationshiD between human T-lvmphotro-MATERIAL AND METHODS phic virus type I (HTLV-I) prov/ral DNA and its expreision in the lymph nodes, HTLV-I DNA and tax/rex mRNA were directly amplified by polymerase chain reaction in situ hybridization (PCRASH), and reverse transcription (RT)-PC
The aim of this study was to evaluate the changes of tumor cell contamination in bone marrow (BM) and peripheral blood (PB) during the clinical course of patients with advanced neuroblastoma by detecting tyrosine hydroxylase (TII) mRNA to clarify the appropriate source and time for harvesting hemato