Detection of human T-lymphotrophic virus type-I DNA and mRNA in the lymph nodes; using polymerase chain reaction in situ hybridization (PCR/ISH) and reverse transcription (RT-PCR/ISH)
✍ Scribed by Koichi Ohshima; Junji Suzumiya; Shuji Izumo; Yasuo Mukai; Kohtaro Tashiro; Masahiro Kikuchi
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- French
- Weight
- 727 KB
- Volume
- 66
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
To examine the relationshiD between human T-lvmphotro-MATERIAL AND METHODS phic virus type I (HTLV-I) prov/ral DNA and its expreision in the lymph nodes, HTLV-I DNA and tax/rex mRNA were directly amplified by polymerase chain reaction in situ hybridization (PCRASH), and reverse transcription (RT)-PCR/ISH [RT-PCR/ ISH]. We studied 24 lymph nodes from patients with adult T-cell leukemia/lymphoma (ATLL), incipient ATLL (I-ATLL), and HTLV-I-associated lymphadenitis dermatopathic type (HAL-D) and enlarged paracortical type (HAL-EP). In ATLL, 40-60% of the nucleated cells were positive for HTLV-I proviral DNA by PCR/ISH, while in I-ATLL and HAL, respectively 5-20% and less than 1-5% of cells were positive. The number of mRNAexpressing cells was smaller than that of the proviral DNApositive cells. The mRNA-expressing cells varied in number among the ATLL and I-ATLL cases, while they were only rarely observed in HAL-D and HAL-EP. These results show that HTLV-I infection and activation might increase with malignant transformation of the target T helper cells.