𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Analysis of acylcarnitines as their N-demethylated ester derivatives by gas chromatography-chemical ionization mass spectrometry

✍ Scribed by Zhi-Heng Huang; Douglas A. Gage; Loran L. Bieber; Charles C. Sweeley

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
827 KB
Volume
199
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A novel approach to the analysis of acylcarnitines has been developed. It involves a direct esterification using propyl chloroformate in aqueous propanol followed by ion-pair extraction with potassium iodide into chloroform and subsequent on-column N-demethylation of the resulting acylcarnitine propyl ester iodides. The products, acyl N-demethylcarnitine propyl esters, are volatile and are easily analyzed by gas chromatography-chemical ionization mass spectrometry. For medium-chain-length (C4-C12) acylcarnitine standards, detection limits are demonstrated to be well below 1 ng starting material using selected ion monitoring. Well-separated gas chromatographic peaks and structure-specific mass spectra are obtained with samples of synthetic and biological origin. Seven acylcarnitines have been characterized in the urine of a patient suffering from medium-chain acyl-CoA dehydrogenase deficiency.

📜 SIMILAR VOLUMES

Analysis of alkenes by copper ion chemic
Analysis of alkenes by copper ion chemical ionization gas chromatography/mass spectrometry and gas chromatography/tandem mass spectrometry
✍ Fordham, P. J.; Chamot-Rooke, J.; Giudice, E.; Tortajada, J.; Morizur, J.-P. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 164 KB 👁 2 views

A novel chemical ionization/fast atom bombardment (CI/FAB) source was used to analyse alkenes by chemical ionization mass spectrometry (CI-MS) using copper ions as the ionizing agent. The Cu Y -CI mass spectra showed abundant pseudomolecular adduct ions [alkene-Cu] Y and characteristic fragment ions

Negative ion chemical ionization gas chr
Negative ion chemical ionization gas chromatography/mass spectrometry and gas chromatography/tandem mass spectrometry of prostanoid pentafluorobenzyl ester/methoxime/trimethylsilyl ether derivatives
✍ Horst Schweer; Hannsjörg W. Seyberth; Claus O. Meese; Otto Fürst 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 526 KB

Negative ion chemical ionization mass spectra of prostaglandin F,,, prostaglandin EZ, prostaglandin D2, 6-0x0prostaglandin F,,, 2,3-dinor-6-oxo-prostaglandin F,, , thromboxane B,, 2,3-dinor-thromboxane B,, and 1 l-dehydrothromboxane B2 pentafluorobenzyl ester (PFB)/methoxime/trimethylsilyl ether der

Analysis of naturally occurring α-acetot
Analysis of naturally occurring α-acetotriacylglycerides by gas chromatography — chemical ionization mass spectrometry
✍ Spitzer, Volker ;Aichholz, Reiner 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 485 KB

## Abstract The seed oils of three Maytenus species have been examined by high temperature GLC using SOP‐50 (methoxy‐terminated) and OV‐225‐OH (OH‐terminated) glasscapillary columns. The main compounds (71–76%) could be identified as α‐mono‐acetotriacylglycerols by a combination of high temperature

Direct analysis by electrospray ionizati
Direct analysis by electrospray ionization and matrix-assisted laser desorption ionization mass spectrometry of standard and urinary acylcarnitines. Comparison with fast atom bombardment and gas chromatography chemical ionization mass spectrometry
✍ Gilbert Briand; Monique Fontaine; Ralf Schubert; Guy Ricart; Pierre Degand; Jose 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 712 KB

## Abstract A simple method using matrix‐assisted laser desorption ionization mass spectrometry (MALDI‐MS) is described for the characterization of acylcarnitines in standard solutions and urinary samples from controls and patients with β‐oxidation deficiency disorders. This mass spectrometric appr