Analysis of naturally occurring α-acetotriacylglycerides by gas chromatography — chemical ionization mass spectrometry

✍ Scribed by Spitzer, Volker ;Aichholz, Reiner

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 485 KB
• Volume: 19
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240190905

✦ Synopsis

Abstract

The seed oils of three Maytenus species have been examined by high temperature GLC using SOP‐50 (methoxy‐terminated) and OV‐225‐OH (OH‐terminated) glasscapillary columns. The main compounds (71–76%) could be identified as α‐mono‐acetotriacylglycerols by a combination of high temperature GLC‐MS in negative chemical ionization mode with a CH~4~/N~2~O mixture as reactant gas and other spectroscopical methods (infrared, ^1^H‐ and ^13^C‐nuclear magnetic resonance spectroscopy). The OV‐225‐OH phase demonstrated better separation properties that the SOP‐50 phase for this substance class and up to eight species of α‐monoacetotriacylglycerols could be separated. The content of usual triacyclglycerols was below 1%. The results of the analysis of the individual fatty acids as their methyl esters by GC and GC‐MS are also presented.