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Analysis of a mutant expressing temperature-sensitive changes of cell size in Tetrahymena

✍ Scribed by Harriet Palissa; Hans-Martin Seyfert; Günter Cleffmann

Book ID
102886007
Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
809 KB
Volume
123
Category
Article
ISSN
0021-9541

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✦ Synopsis

A temperature-sensitive mutant of Tetrahymena expresses an increase in cell volume by a factor of 2.5 upon shift to restrictive temperature. Cellular amounts of protein, RNA, and DNA increase at roughly the same proportions. The mutant cell size i s attained by cessation of divisions immediately after temperature shift for a period of time which is about equal to one generation time. During this time cell growth and DNA replication continue at virtually unchanged rates. Maintained at the restrictive temperature the mutant cells divide at the same rate as t h e wild-type cells. Upon return to t h e permissive temperature, cell size is reduced by t h e combined effects of an accelerated division rate together with a decelerated growth rate.

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