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Analysis of 5-Methylcytosine in DNA of Breast and Colon Cancer Tissues

✍ Scribed by Anna-Maria Barciszewska; Dawid Murawa; Iwona Gawronska; Pawel Murawa; Stanislaw Nowak; Miroslawa Z. Barciszewska

Book ID
111741953
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
301 KB
Volume
59
Category
Article
ISSN
1521-6543

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Analysis of 5-methylcytosine in DNA: I.
Analysis of 5-methylcytosine in DNA: I. Mass spectrometry
✍ Joseph Deutsch; Aharon Razin; John Sedat πŸ“‚ Article πŸ“… 1976 πŸ› Elsevier Science 🌐 English βš– 419 KB

A method is described for the unambiguous rapid identification and quantitation of the minor base, 5-methylcytosine, in DNA using high resolution mass spectrometry. This method can detect one 5-methylcytosine residue per 5500 nucleotides in +x 174 DNA, in a sample of less than 10 pg and requires les

Analysis of 5-methylcytosine in DNA: II.
Analysis of 5-methylcytosine in DNA: II. Gas chromatography
✍ Aharon Razin; John Sedat πŸ“‚ Article πŸ“… 1977 πŸ› Elsevier Science 🌐 English βš– 454 KB

A method for analyzing 5methylcytosine in DNA by gas chromatography is described. The method is based on degradation of the DNA to its free bases by treatment with trifluoroacetic acid and gas chromatography of the trimethylsilyl derivatives of the free bases. Chromatography of microgram amounts of