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Analysis by immunoblotting of human cytomegalovirus antibody in sera of renal transplant recipient

✍ Scribed by Keiko Shimokawa; Tsugiya Murayama; Shigeo Ishida; Toru Furukawa

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
490 KB
Volume
21
Category
Article
ISSN
0146-6615

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✦ Synopsis

Human cytomegalovirus-infected cell polypeptides were immunoreacted by sera of renal transplant recipients and compared with those reactive with sera of healthy adult donors by means of the Western immunoblotting technique. At least 15 polypeptides with molecular weights of 155K, 123K, 102K, 89K, 79K, 71K, 65K, 60K, 55K, 50K, 46K, 42K, 38K, 33K, and 28K were immunoreacted. Sera obtained serially from renal transplant recipients reacted with most of these polypeptides and reacted more frequently and intensely with the smaller polypeptide species such as 38K, 33K, and 28K, compared with sera of healthy seropositive adults. The implications of these findings are discussed.

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