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An Optimization Study of a pH-Inducible Promoter System for High-Level Recombinant Protein Production in Escherichia coli

✍ Scribed by K. Y. SAN; G. N. BENNETT; C.-H. CHOU; A. A. ARISTIDOU

Book ID: 119871116
Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 396 KB
Volume: 721
Category: Article
ISSN: 0890-6564
DOI: 10.1111/j.1749-6632.1994.tb47400.x

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## Abstract Extracellular production of recombinant proteins in __Escherichia coli__ has several advantages over cytoplasmic or periplasmic production. However, nonpathogenic laboratory strains of __E. coli__ generally excrete only trace amounts of proteins into the culture medium under normal grow