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An NADH coupled assay system for galactose oxidase

✍ Scribed by Gad Avigad

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
434 KB
Volume
86
Category
Article
ISSN
0003-2697

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✦ Synopsis

A galactose oxidase (EC 1.1.3.9):NADH-peroxidase (EC 1.11.1.1) coupled assay system is used for the estimation of galactose oxidase activity. Spectrophotometric measurement of NADH consumption yields direct quantitative value of enzymic activity or can be used for the end-point determination of the amount of galactose oxidase substrate present in test solutions. Use of similar coupled systems is suggested for the assay of other H,O,-producing enzymes and their substrates.

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