✦ LIBER ✦
An NADH-coupled assay for femtogram or nanogram quantities of chymotrypsin
✍ Scribed by Edmund A. Mroz; Claude Lechene
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 371 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Chymotrypsin can be determined with an NADH-coupled assay. Hydrolysis of the substrate benzoyhyrosine ethyl ester is monitored by coupling the liberation of ethanol to the production of NADH and determining the NADH spectrophotometrically or fluorometrically. Nanogram quantities of chymotrypsin can be determined in milliliter volumes. With these microfluorescence methods this assay can be performed in a final volume of less than a nanoliter, allowing determination of femtogram quantities of chymotrypsin, the amount present in an individual zymogen granule.