The development of new catalytic methods, in particular enzymatic processes, is increasingly being followed with combinatorial and evolutionary methods, whereby sensitive assays for catalysis play an essential role. [1Β±3] Most enzyme assays are based on chromogenic or fluorogenic substrates. [1,4, 5
An in-gel enzyme assay
β Scribed by Jozsef Szeberenyi
- Publisher
- The American Society for Biochemistry and Molecular Biology
- Year
- 2004
- Tongue
- English
- Weight
- 47 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1470-8175
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The development of new catalytic methods, in particular enzymatic processes, is increasingly being followed with combinatorial and evolutionary methods, whereby sensitive assays for catalysis play an essential role. [1Β±3] Most enzyme assays are based on chromogenic or fluorogenic substrates. [1,4, 5
A sensitive assay highly specific for horseradish peroxidase has been developed. The basic principle of the procedure is similar to that of the enzyme-linked immunosorbent assay technique. Sensitivity is the range 0.156-2.5 ng/ml. Background activity in undiluted plasma and lymph under these conditi
A new and novel method for determination of serum acetaminophen is described. The assay, which can be completed in less than 5 min, is based on the enzymatic hydrolysis of acetaminophen, with subsequent calorimetric detection of the aminophenol so produced. Various possible means of aminophenol esti
UDP-Gal:Galbeta1-4GlcNAc alpha1,3-galactosyltransferase (alpha3GalT) is responsible for the synthesis of carbohydrate xenoantigen Galalpha1-3Galbeta1-4GlcNAc. In this work a convenient and sensitive assay system for quantification of alpha3GalT activity by enzyme-linked lectin assay (ELLA) with colo