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An improved spectrophotometric method for the determination of free, bound and total N-acetylneuraminic acid in biological fluids

✍ Scribed by Maria-Helen E. Spyridaki; Panayotis A. Siskos

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
668 KB
Volume
327
Category
Article
ISSN
0003-2670

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✦ Synopsis

The spectrophotometric periodate-resorcinol method for the measurement of free, bound and total N-acetylneuraminic acid has been modified. The proposed method improves sensitivity by 27%, reduces the time of analysis by 33-52% and addresses interferences from biological substances. The improved procedure was applied to serum and urine samples for the determination of free, bound and total N-acetylneuraminic acid and compared successfully to the method reported by Jourdian et al. and to a commercially available enzymatic method. The results show that the proposed method is reliable, simple and convenient for routine use in clinical laboratory.

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